Journal: BMC Microbiology

Article Title: A novel method for investigating Burkholderia cenocepacia infections in patients with cystic fibrosis and other chronic diseases of the airways

doi: 10.1186/s12866-016-0811-7

Figure Lengend Snippet: The effect of B. cenocepacia infection on the integrity of HLCC monolayers. Confluent HLCCs were subjected to Ham’s F-12 complete medium ( a and b ) or 60 % ASMDM ( c and d ), infected with B. cenocepacia at MOI 0.3-5 ( b and d ) or mock-infected with saline ( a and c ), and incubated at 37 °C (7 % CO 2 ). After 24 h, HLCC monolayers were assessed for damage with an inverted Zeiss axiovert 40 CFL microscope (1000X magnification). HLCC monolayers in both mock-infected wells are confluent with no damage ( a and c ). HLCC monolayer in Ham’s F-12 complete medium with B. cenocepacia shows no sign of damage, although a dense mass is floating in the well. Because this is seen in infected wells only, we think the aggregations are clouds of bacterial cells floating in the supernatant ( arrows ) ( b ). HLCC monolayer in 60 % ASMDM with B. cenocepacia shows damage as indicated by gaps in the monolayer ( black stars ). Clouds of bacterial cells can be seen as well ( arrows ) ( d )

Article Snippet: After 24 h, damage to the monolayer was assessed with an inverted Zeiss axiovert 40 CFL microscope (W. Nuhsbaum, Inc., McHenry, IL), a microscope-mounted camera (AmScope, Irvine, CA), and Leica LAS v4.6.2. software.

Techniques: Infection, Incubation, Microscopy